- Research
- Open Access

# Analysis of stability and bifurcations of fixed points and periodic solutions of a lumped model of neocortex with two delays

- Sid Visser
^{1, 2}Email author, - Hil GE Meijer
^{1, 2}, - Michel JAM van Putten
^{2, 3}and - Stephan A van Gils
^{1, 2}

**2**:8

https://doi.org/10.1186/2190-8567-2-8

© Visser et al.; licensee Springer 2012

**Received: **4 October 2011

**Accepted: **18 March 2012

**Published: **25 April 2012

## Abstract

A lumped model of neural activity in neocortex is studied to identify regions of multi-stability of both steady states and periodic solutions. Presence of both steady states and periodic solutions is considered to correspond with epileptogenesis. The model, which consists of two delay differential equations with two fixed time lags is mainly studied for its dependency on varying connection strength between populations. Equilibria are identified, and using linear stability analysis, all transitions are determined under which both trivial and non-trivial fixed points lose stability. Periodic solutions arising at some of these bifurcations are numerically studied with a two-parameter bifurcation analysis.

## Keywords

## 1 Introduction

Epilepsy is a neurological disease characterized by an increased risk of recurring seizures that affects about 1% of the world population. Such seizures typically manifest themselves as brief periods in which neural activity is more synchronized than a certain baseline level. In lumped models of neural activity in the brain, these seizures are, for that reason, often characterized as large-amplitude oscillations [1]. Many causes might exist for the neural network to start oscillating, e.g., a slow parameter or an external factor might cause a bifurcation [2], or a perturbation might force the system to a different attractor [3].

Small Hopfield networks of this and similar forms have been studied in detail by various researches [6–22]. For example, Olien and Bélair [16] studied a two-node network with both delayed feedforward and delayed feedback connections between the nodes. Later, the same model was analyzed further by Wuan and Rei [18]. The delays in this model, however, are node-specific (the delays for all outgoing connections of a node are unique for that node) instead of connection-specific (the delays are unique for each type of connection: excitatory and inhibitory). The latter case applies to our network.

We particularly notice the work by Shayer and Campbell [17] that studies a model very similar to the system (Equation 1) except for the fact that they choose the activation functions as odd functions. Although they numerically identify multi-stability of steady states and a periodic solution, their study mainly focuses on analytical determination of the stability and bifurcations of the trivial equilibrium in terms of the time lag parameters. In 2005, Campbell *et al.* studied the numerical continuation of periodic solutions in a ring of neurons [9]. We will extend a similar approach to a two-parameter bifurcation study in this work.

Because Hopfield networks originate from computer science to solve mathematical programming problems [23], it is more common to study models of the Wilson-Cowan type for physiological modeling [24]. On that note, we like to point to a study by Coombes and Laing of a Wilson-Cowan type model, which is very similar to our model, in which they observe a variety of steady states, periodic solutions and chaos [25]. While Hopfield models are uncommon in mathematical neuroscience, we are not the first to study these models with a physiological relevance. For instance, Song *et al.* studied two clusters, each consisting of an excitatory and an inhibitory node that projected onto each other with delayed connections [26]. They assumed that the connections between the nodes could be faster in one direction than in the other, and they studied the model’s dependency on this difference in time lags. Furthermore, they are, to our knowledge, the only group that has performed a numerical bifurcation study of periodic orbits in two parameters for this type of model.

Due to the physiological background of our model, the delays are known and we consider fixed values of ${\tau}_{i}$ and ${\tau}_{e}$. Because of that, we are primarily interested in the parameters related to connection strength as these may be amended with anti-epileptic drugs. Although these results will depend on the chosen values of the delays, we elaborate on their robustness under variations of these delays in the discussion.

for certain *S* that is smooth, strictly increasing and satisfies $S(0)=0$ and ${S}^{\prime}(0)=1$. Typically, $S(x)$ is bounded and sigmoidal, *i.e.* *S* has exactly one inflection point. The results in Section 2 are independent of the specific shape of *S*, but we will specify *S* for the numerical bifurcation analysis.

In the following section, we will study this system analytically by determining its fixed points and the linear stability of these points. We will identify a stability region in parameter space and classify the bifurcations on the edge of this region. For Hopf bifurcations of the trivial steady state, we compute the first Lyapunov coefficient to study the criticality of these bifurcations. In the ‘Numerical bifurcation analysis’ section, we use software packages to determine (numerically) how the presence and stability of the bifurcating periodic solutions depend on the parameters ${\alpha}_{1}$ and ${\alpha}_{2}$.

## 2 Equilibria: linear stability and bifurcations

In this section, we study the equilibria as well as their linear stability. Necessary conditions for saddle-node, trans-critical and Hopf bifurcations are derived. Thereafter, the first Lyapunov coefficient is evaluated for the Hopf bifurcations to determine their criticality.

### 2.1 Equilibria and stability region

First we note, since $S(0)=0$, that the origin $({x}_{1},{x}_{2})=(0,0)$ is always a fixed point of the system (Equation 4). For the non-trivial fixed points, the following holds:

**Theorem 1**

*The system*(

*Equation*4)

*admits exclusively symmetric fixed points*:

*Proof*First we note that, since $S(x)$ is a continuous strictly increasing function, its inverse function ${S}^{-1}(x)$ exists, and it is also continuous and strictly increasing. Next define:

Because of monotonicity of both *S* and ${S}^{-1}$ and positiveness of all parameters, *H* is continuous and strictly increasing as well.

*H*on both sides of this inequality and use of the conditions in Equation 5 yield:

This contradicts our assumption; hence, we conclude that ${x}_{1}^{\ast}={x}_{2}^{\ast}={x}^{\ast}$. □

Both ${k}_{1}$ and ${k}_{2}$ take positive values only because ${S}^{\prime}$ is positive as well as the parameters ${\alpha}_{i}$ and ${\beta}_{i}$ for $i=1,2$.

**c**exist if the characteristic equation is satisfied:

The spectra of linear DDEs with two delays, like Equations 10a and 10b, have been studied extensively since the 1960s (for instance, Bellman, Cooke and Hale [27, 28]). The main consensus of these works is that the stability region often has a complex shape in terms of the parameters of the differential equation. The majority of the results in the remainder of this section and the next one (*i.e.* ‘Bifurcations’ section) could be considered as ‘common knowledge’. For the purpose of clarity, however, we have chosen to present a short derivation of these results.

We start by denoting the following theorem regarding symmetry of solutions:

**Theorem 2** *Roots of*${\mathrm{\Delta}}_{-}$*correspond to symmetric solutions*, *whereas roots of*${\mathrm{\Delta}}_{+}$*relate to asymmetric solutions*.

*Proof*Let ${\mathbb{Z}}_{2}$ act on ${\mathbb{R}}^{2}$ so that $-1\in {\mathbb{Z}}_{2}$ acts as $\xi (x,y):(x,y)\mapsto (y,x)$, then:

□

Using the characteristic equation, we can find a relation between the parameters $({k}_{1},{k}_{2})$ and the eigenvalues:

**Theorem 3**

*Let*$\lambda =\rho +i\omega $

*for*$\rho ,\omega \in \mathbb{R}$

*satisfy the characteristic equation*(

*Equation*9)

*and let*${\tau}_{2}>{\tau}_{1}>0$,

*then the following inequality holds*:

*Proof*Solutions of the characteristic equation (Equation 9) satisfy either ${\mathrm{\Delta}}_{+}(\lambda )=0$ or ${\mathrm{\Delta}}_{-}(\lambda )=0$. Upon assuming ${\mathrm{\Delta}}_{+}(\lambda )=0$, it follows that:

which yields the inequality (Equation 12). A similar argument for ${\mathrm{\Delta}}_{-}(\lambda )$ yields the same inequality. □

**Corollary 4** *An equilibrium of the system* (*Equation* 4) *is asymptotically stable if*$|{k}_{1}|+|{k}_{2}|<1$.

*Proof*The inequality (Equation12) yields in this case:

which can only hold for $\rho <0$. Therefore, all roots of the characteristic matrix have a negative real part and the equilibrium is asymptotically stable. □

Having obtained a minimal stability region in the Corollary 4, we study conditions for bifurcations of equilibria to expand the minimal stability region determined by Corollary 4.

### 2.2 Bifurcations

The stability of an equilibrium of a DDE is lost when one or more eigenvalues pass through the origin or the imaginary axis. The first case, in which a real eigenvalue crosses through the origin, is characterized in the following theorem:

**Theorem 5** *The linearized system* (*Equation* 6) *has at least one zero eigenvalue if and only if*$1+{k}_{1}+{k}_{2}=0$*or*$1+{k}_{1}-{k}_{2}=0$.

*Proof*Substitution of $\lambda =0$ into the characteristic equation (Equation 9) yields that either ${\mathrm{\Delta}}_{+}(0)=0$ or ${\mathrm{\Delta}}_{-}(0)=0$ and hence:

□

Since the origin is always a fixed point of the system, the conditions in Theorem 5 correspond to transcritical bifurcations. For non-trivial fixed points, these conditions imply either a fold bifurcation or a trans-critical bifurcation. Because ${k}_{1}$ and ${k}_{2}$ are both positive, saddle-node bifurcations from ${\mathrm{\Delta}}_{+}$ cannot occur. This, in combination with Theorem 2, leads to the conclusion that no symmetry-breaking steady-state bifurcations exist, a result which we also obtained in Theorem 1.

The case in which a pair of complex eigenvalues passes the imaginary axis is summarized in the following theorem:

**Theorem 6** *Two piecewise continuous functions*${\mathbf{h}}_{+}(\omega )$*and*${\mathbf{h}}_{-}(\omega )$*exist in parameter space*$({k}_{1},{k}_{2})$*for which the characteristic equation* (*Equation* 9) *has a pair of purely imaginary roots*$\lambda =\pm i\omega $. *Furthermore*, *when*$\omega =-tan\omega {\tau}_{1}=-tan\omega {\tau}_{2}$, *a line*${k}_{1}+\sigma {k}_{2}=c$*exists for some* *c* *and*$\sigma =\pm 1$*for which Equation* 9*has roots*$\pm i\omega $.

*Proof*Substituting $\lambda =i\omega $ with $\omega >0$ into Equation 9 yields that either ${\mathrm{\Delta}}_{+}(i\omega )=0$ or ${\mathrm{\Delta}}_{-}(i\omega )=0$. The roots of ${\mathrm{\Delta}}_{+}(i\omega )$ are considered first:

*ω*by matrix inversion:

*A*being the matrix in Equation 15, follows that:

for $\sigma =\pm 1$ such that $cos\omega {\tau}_{1}=\sigma cos\omega {\tau}_{2}$.

Furthermore, the same line of solutions and corresponding condition as in Equations 18a and 18b hold for ${\mathrm{\Delta}}_{-}(i\omega )$. For a Hopf bifurcation to occur, any of the equations (Equations 16 to 19) must be satisfied. □

In Theorem 2, we have already shown that Hopf bifurcations caused by ${\mathrm{\Delta}}_{-}$ correspond to symmetric periodic solutions. For Hopf bifurcations induced by ${\mathrm{\Delta}}_{+}$, the following holds:

**Theorem 7** *Hopf bifurcations corresponding with*${\mathrm{\Delta}}_{+}$*yield asymmetric periodic solutions*, *i*.*e*., ${x}_{1}(t)={x}_{2}(t+\frac{1}{2}T)$*with* *T* *the period of the solution*.

*Proof* Let $\lambda =i{\omega}_{0}$ for ${\omega}_{0}>0$ be a simple root of ${\mathrm{\Delta}}_{+}$ (*i.e.*, of algebraic multiplicity one) and *p* a corresponding eigenvector of $\mathrm{\Delta}(i{\omega}_{0})$. Then, from Hopf bifurcation theory, we know that, for *ϵ*, sufficiently small ${C}^{1}$ functions ${\mathbf{k}}^{\ast}(\u03f5)$, ${\omega}^{\ast}(\u03f5)$ and ${\mathbf{x}}^{\ast}(\u03f5)$ exist, taking values in ${\mathbb{R}}^{2}$, $\mathbb{R}$ and $C([-h,0],{\mathbb{R}}^{2})$, respectively. Furthermore, ${\mathbf{k}}^{\ast}(\u03f5)\to {\mathbf{h}}_{+}({\omega}_{0})$, ${\omega}^{\ast}(\u03f5)\to {\omega}_{0}$ and $\mathbf{x}(\u03f5)(t)=\u03f5\mathrm{\Re}({e}^{i{\omega}^{\ast}(\u03f5)t}p)+o(\u03f5)$ for $\u03f5\downarrow 0$. For $\mathbf{k}={\mathbf{k}}^{\ast}(\u03f5)$ and *ϵ*, sufficiently small $\frac{2\pi}{{\omega}^{\ast}(\u03f5)}$-periodic solutions $\mathbf{x}(t)={\mathbf{x}}^{\ast}(\u03f5)(t+\theta )$ exist with $\theta \in [0,2\pi /{\omega}^{\ast}(\u03f5))$.

*ξ*, it follows that the bifurcating periodic solution inherits this symmetric property:

So, the condition for asymmetric periodic solutions is satisfied. □

Studying the right diagram of Figure 2, we observe that the bifurcation curves do not coincide with the bounds of the stability region from Corollory 4. Hence, it appears that parameters exist outside this square stability region for which it still holds that all eigenvalues have negative real part. We now determine the full stability region around the origin of the $({k}_{1},{k}_{2})$-plane by showing that instabilities are exclusively induced by low frequencies. More precisely:

**Theorem 8** *The square*$|{k}_{1}|+|{k}_{2}|<\sqrt{1+{\omega}_{0}^{2}}$*contains no eigenvalues*$\lambda =\pm i\omega $*for*$\omega \ge {\omega}_{0}>0$.

*Proof* This follows from substitution of $\lambda =i{\omega}_{0}$ into Theorem 3 and the fact that $\sqrt{1+{\omega}_{0}^{2}}$ is a monotically increasing function. □

So, if we choose ${\omega}_{0}$ sufficiently large as dictated by Theorem 8, no other bifurcations are located inside the bifurcation diagrams of Figure 2 for $\omega >{\omega}_{0}$. Hence, we can extend the stability region from the square region to the nearest bifurcation. This new stability region is hatched in the right diagram of Figure 2.

Since we are mainly interested in stable solutions, we consider only bifurcation curves that bound the stability region. Even though we identified a bounded stability region in parameter space, we cannot assure that this is the only region in which fixed points are stable. As shown in [29], the roots of either ${\mathrm{\Delta}}_{-}$ or ${\mathrm{\Delta}}_{+}$ can contain multiple, disjoint regions in parameter space in which all roots have negative real parts. Since in our case, however, the eigenvalues of Equation 6 are the union of the eigenvalues of the Equations 10a and 10b, we conjecture that no other stable regions exist in parameter space than the one shown in Figure 2.

*ω*for which these curves bound the stability region by ${\mathrm{\Omega}}_{S}({\mathbf{h}}_{+})$ and ${\mathrm{\Omega}}_{S}({\mathbf{h}}_{-})$, respectively. We compute approximations of these ranges:

It follows from Equation 7 that, for the trivial equilibrium, the bifurcation diagram in the $({k}_{1},{k}_{2})$-plane determines the bifurcation diagram in the $({\alpha}_{1},{\alpha}_{2})$-plane up to linear rescaling.

### 2.3 The first Lyapunov coefficient

Hopf bifurcations give rise to either stable or unstable periodic solutions depending on the criticality. Therefore, we determine the first Lyapunov coefficient. Since it is easier to relate ${k}_{1}$ and ${k}_{2}$ to ${\alpha}_{1}$ and ${\alpha}_{2}$ in the origin than at non-trivial fixed points, we only consider Hopf bifurcations at the origin.

*p*and

*q*be eigenvectors of the characteristic matrix $\mathrm{\Delta}(i\omega )$ and ${\mathrm{\Delta}}^{\ast}(i\omega )$, respectively. We normalize these vectors such that ${q}^{T}{\mathrm{\Delta}}^{\prime}(i\omega )p=1$. By choosing $p={[1,1]}^{T}$ as an eigenvector of $\mathrm{\Delta}(i\omega )$

*q*takes the form:

**f**is symmetric,

*i.e.*${\mathbf{f}}_{j}([x,x])=f(x)$ for $j=1,2$, and that it does not contain any cross terms, that is $\frac{{\partial}^{2}}{\partial {x}_{1}\phantom{\rule{0.2em}{0ex}}\partial {x}_{2}}\mathbf{f}([{x}_{1},{x}_{2}])=0$. Therefore, both components of the differential operators ${D}^{2}\mathbf{f}([x,x])$ and ${D}^{3}\mathbf{f}([x,x])$ will be identical when evaluated for symmetric arguments and we denote these components by ${f}^{\u2033}(x)$ and ${f}^{\u2034}(x)$, respectively. By using the multi-linear properties of the operators, we expand ${c}_{1}$:

As the real part of this expression is too intricate to study analytically, we study the first Lyapunov coefficient only numerically.

Such a root corresponds with a generalized Hopf bifurcation at which the criticality of the Hopf bifurcation changes. Hence, for $\omega <0.281$, the Hopf bifurcations are supercritical and for $\omega >0.281$ the bifurcations are subcritical.

So far, we have studied the fixed points and their bifurcations extensively, and we have shown that the system can exhibit stable periodic solutions. Since the further development of these periodic solutions cannot be studied with a local analysis of points, we must use a different approach to continue this study. Therefore, we explore the behavior of the periodic solutions numerically in the next section.

## 3 Numerical bifurcation analysis

Here, we investigate the outcome of the periodic solutions that emanate from the Hopf bifurcations in the above text. We turn to a numerical analysis since the orbits cannot be determined analytically. More specifically, we use dde-biftool[31] to study non-trivial fixed points, and for continuation of periodic solutions, we use Knut [32]. In the following analysis, we only describe branches of solutions that are by some means associated with stable solutions. Branches not resulting in stable solutions are not discussed further.

### 3.1 One parameter bifurcations in ${\alpha}_{2}$

First, a bifurcation analysis is done in a single parameter. Here, we have chosen to vary the parameter ${\alpha}_{2}$ that represents the total amount of excitation in the system. The inhibition ${\alpha}_{1}$ is fixed at 0.069, and the function *S* is chosen as Equation 28 with $a=1$.

Overview of approximate parameter values for codim-1 bifurcations in ${\alpha}_{2}$

Point | ${\alpha}_{2}$ |
---|---|

${H}_{1}$ | 0.771 |

${B}_{1}$ | 0.948 |

${F}_{1}$ | 0.5211 |

${H}_{2}$ | 0.5212 |

${H}_{3}$ | 0.5212 |

${H}_{4}$ | 1.052 |

${\mathit{PD}}_{1}$ | 0.650 |

${\mathit{LPC}}_{1}$ | 0.462 |

${\mathit{PD}}_{2}$ | 0.465 |

${\mathit{PD}}_{3}$ | 0.596 |

${\mathit{LPC}}_{2}$ | 0.615 |

${\mathit{PD}}_{4}$ | 0.522 |

${\mathit{LPC}}_{3}$ | 0.464 |

${\mathit{LPC}}_{4}$ | 0.619 |

#### 3.1.1 Fixed points

The origin is a natural starting point of our discussion of the bifurcation analysis because it is always a fixed point of Equation 4. The origin is stable until it undergoes a subcritical Hopf bifurcation ${H}_{1}$. Thereafter, it goes through two other Hopf bifurcations and a branch point ${B}_{1}$. For this value of ${\alpha}_{1}$, these Hopf bifurcations involve only unstable periodic solutions.

Next, we follow the fixed point that emerges from the branch point ${B}_{1}$. This fixed point encounters numerous Hopf bifurcations until it reaches a fold bifurcation ${F}_{1}$. Thereafter, it rapidly undergoes two distinct subcritical Hopf bifurcations: ${H}_{2}$ and ${H}_{3}$, becoming stable at ${H}_{3}$. Continuing the intersecting fixed point at ${B}_{1}$ in the other direction, the steady state goes through two Hopf bifurcations until it gains stability at the subcritical Hopf bifurcation ${H}_{4}$ (not shown).

Whether a Hopf bifurcation is caused by a crossing of ${\mathrm{\Delta}}_{-}$ or ${\mathrm{\Delta}}_{+}$ determines whether this Hopf bifurcation results in symmetric or asymmetric periodic solutions. Hence, we conclude that Hopf bifurcations ${H}_{1}$, ${H}_{2}$ and ${H}_{4}$ yield symmetric periodic solutions, and that ${H}_{3}$ yields asymmetric ones.

#### 3.1.2 Periodic solutions

Next, we investigate the periodic solutions emanating from the Hopf bifurcations ${H}_{1}$, ${H}_{2}$ and ${H}_{3}$. The branch of unstable periodic solutions that emerges from ${H}_{1}$ consists of symmetric solutions. This matches with the analytical results since ${H}_{1}$ lies on ${\mathbf{h}}_{-}$ and it, therefore, corresponds with symmetric solutions. The branch subsequently goes through a subcritical Neimark-Sacker bifurcation (not shown), a supercritical period-doubling bifurcation ${\mathit{PD}}_{1}$, a limit point of cycles ${\mathit{LPC}}_{1}$ and a subcritical period-doubling bifurcation ${\mathit{PD}}_{2}$ at which it finally becomes stable. Then, the solution remains stable until it undergoes a supercritical period doubling bifurcation ${\mathit{PD}}_{3}$, folds over in ${\mathit{LPC}}_{2}$, goes through a subcritical period doubling bifurcation ${\mathit{PD}}_{4}$ and terminates in the Hopf bifurcation ${H}_{2}$.

Solutions branching from ${\mathit{PD}}_{1}$ are asymmetric. This branch folds over near ${\mathit{PD}}_{1}$ and a second time at ${\mathit{LPC}}_{3}$ where it gains stability. Following this branch, stability is lost at ${\mathit{LPC}}_{4}$ and it ends in Hopf bifurcation ${H}_{3}$. We mention a branch sprouting from ${\mathit{PD}}_{3}$ of symmetric solutions that is initially stable but then folds over three times before it terminates in ${\mathit{PD}}_{4}$. Even though these solutions are initially stable, we have been unable to find these solutions in simulations because their domain of attraction is relatively small.

#### 3.1.3 Summary

### 3.2 Two parameter bifurcations in ${\alpha}_{1}$ and ${\alpha}_{2}$

Overview of approximate parameter values for codim-2 bifurcations in ${\alpha}_{1}$ and ${\alpha}_{2}$

Point | ${\alpha}_{2}$ | ${\alpha}_{1}$ |
---|---|---|

${\mathit{HH}}_{1}$ | 0.829 | 0.028 |

${\mathit{ZH}}_{1}$ | 0.840 | 0.004 |

${\mathit{GH}}_{1}$ | 0.512 | 0.246 |

${\mathit{ZH}}_{2}$ | 0.440 | 0.008 |

${\mathit{FF}}_{1}$ | 0.455 | 0.158 |

| 0.460 | 2.9e-4 |

${\mathit{FF}}_{2}$ | 0.460 | 2.8e-4 |

| 0.460 | 2.9e-4 |

${\mathit{FF}}_{3}$ | 0.460 | 2.8e-4 |

${\mathit{CP}}_{1}$ | 0.421 | 0.390 |

${\mathit{CP}}_{2}$ | 0.460 | 2.8e-4 |

${\mathit{CP}}_{3}$ | 0.481 | 0.168 |

| 0.531 | 0.114 |

${\mathit{CP}}_{4}$ | 0.460 | 0 |

#### 3.2.1 Steady states

In the one-parameter analysis, we have found that the origin and the non-trivial steady state turn unstable at Hopf bifurcations ${H}_{1}$ and ${H}_{3}$, respectively. Continuing ${H}_{1}$ in two parameters yields a Hopf bifurcation curve, and on this curve, we find a Hopf-Hopf bifurcation ${\mathit{HH}}_{1}$. Following the second Hopf branch (${H}_{5}$) involved, we find a transcritical-Hopf point ${\mathit{ZH}}_{1}$ as it collides with ${B}_{1}$. This corresponds with the analysis of ‘Bifurcations’ section where we showed the existence of zero-Hopf and Hopf-Hopf points (see Equations 22 and 23). The arrangement of these curves is the same as in Figure 3 except for scaling. Since all involved Hopf curves at the points ${\mathit{HH}}_{1}$ and ${\mathit{ZH}}_{1}$ are subcritical, it follows then from the normal form analysis [33] that, for these parameters, no extra stable solutions exist near these points.

Our analysis of the first Lyapunov coefficient also revealed the existence of a generalized Hopf bifurcation (see Equation 29). We numerically identify this point ${\mathit{GH}}_{1}$ along the branch of ${H}_{1}$ by finding an emanating branch of limit point of cycles ${\mathit{LPC}}_{1}$ with Knut. When the Hopf bifurcation ${H}_{3}$ of the non-trivial equilibrium is followed, a zero-Hopf bifurcation ${\mathit{ZH}}_{2}$ is found as ${H}_{3}$ collides with fold bifurcation ${F}_{1}$. We remark that the curves ${H}_{3}$ and ${F}_{1}$ are undistinguishable in the diagram since they are close to each other for all $({\alpha}_{1},{\alpha}_{2})$ considered. The bifurcation ${\mathit{ZH}}_{2}$ is a simple case ([33], $s=1$$\theta >0$), yielding no additional stable solutions. These curves and the corresponding stability regions are shown in Figures 7 and 8. Bi-stability is indicated by the overlapping, darker region.

#### 3.2.2 Symmetric periodic solutions

The stability region of the symmetric periodic solutions is bounded by ${\mathit{PD}}_{2}$ and ${\mathit{PD}}_{3}$. Continuation of ${\mathit{PD}}_{2}$ for stronger inhibition reveals a fold-flip bifurcation ${\mathit{FF}}_{1}$ where the period doubling bifurcation hits ${\mathit{LPC}}_{1}$ branch. Thereafter, it bends away and terminates. Continuing the ${\mathit{LPC}}_{1}$ curve in the same direction, we first find a cusp point ${\mathit{CP}}_{1}$ after which the curve ends in the generalized Hopf bifurcation ${\mathit{GH}}_{1}$. When ${\mathit{PD}}_{2}$ is continued in the other direction (less inhibition), it undergoes a 1:2-resonance bifurcation *R* 2_{1} (*i.e.*, the period doubling branch encounters a period-doubling), and thereafter, it is subjected to a fold-flip bifurcation ${\mathit{FF}}_{2}$ with ${\mathit{LPC}}_{1}$. Following the ${\mathit{LPC}}_{1}$ curve at ${\mathit{FF}}_{2}$, we encounter another fold-flip bifurcation ${\mathit{FF}}_{3}$ and a cusp bifurcation ${\mathit{CP}}_{2}$. At this cusp point, the branch merges with ${\mathit{LPC}}_{2}$.

The branch ${\mathit{PD}}_{3}$ does not undergo any bifurcation when continued for stronger inhibition. Continuation in the other direction reveals a 1:2-resonance bifurcation *R* 2_{2} and the previously identified fold-flip bifurcation ${\mathit{FF}}_{3}$. Unfolding the 1:2-resonance bifurcations *R* 2_{1} and *R* 2_{2} reveals that both points are connected by the curve ${\mathit{NS}}_{1}$ of Neimark-Sacker bifurcations. Therefore, this curve is also part of the boundary of the stability region of symmetric periodic solutions. From the unfolding of these 1:2-resonance bifurcations, we know that branches of stable homoclinic orbits should exist. However, we have been unable to continue these branches.

#### 3.2.3 Asymmetric periodic solutions

From single parameter continuation, it follows that stable asymmetric oscillations are bounded by ${\mathit{LPC}}_{3}$ and ${\mathit{LPC}}_{4}$. Continuation of ${\mathit{LPC}}_{3}$ yields a cusp point ${\mathit{CP}}_{3}$ and a 1:1-resonance bifurcation *R* 1_{1} at which the branch becomes unstable. Hereafter, the stability region is bounded by a branch of Neimark-Sacker bifurcation that sprouts from *R* 1_{1}. When ${\mathit{LPC}}_{3}$ is continued in the other direction, it undergoes a cusp bifurcation (${\mathit{CP}}_{4}$) at ${\alpha}_{1}=0$ where ${\mathit{LPC}}_{3}$ merges with ${\mathit{LPC}}_{4}$.

#### 3.2.4 Summary

With the two-parameter bifurcation analysis, we find that a large part of parameter space corresponds with multi-stability. In the center, we find a region with four different stable solutions: two steady states and two periodic solutions. Furthermore, it can be seen that steady states destabilize for strong values of inhibitory feedback (${\alpha}_{1}$ large) since only periodic solutions exist in the upper part of the bifurcation diagram.

### 3.3 Comparison with a realistic model

*et al.*analyzed a small model of neocortex consisting of 656 neurons to study emergent epileptiform activity [4]. For similar reasons as in this study, they varied only parameters related to excitatory and inhibitory synaptic strength, and they then obtained Figure 9. In this figure, the behavior of their realistic model for different choices of parameters is categorized in one of five categories: desynchronized, irregular bursting, oscillatory, regular bursting and saturated activity. The small exemplary time series show for each class the characteristic behavior of the model except for saturated activity. This latter state is best described as a state in which all neurons are non-stop activated in an incoherent manner.

In the regular bursting state, the model is rather quiet apart from a burst of activity that occurs regularly about every second. These bursts are primarily generated by slow dynamical processes in the underlying neurons. In the absence of slow processes, the network would exhibit no activity in this state [4]. Hence, this type of behavior should be compared with the trivial steady state of our model. Furthermore, the non-trivial steady state in our simplified model corresponds with saturated activity in the detailed model because the network is very active, but no clear oscillations or rhythms are observed. Finally, the oscillatory state can be compatible with both the symmetric and asymmetric periodic solutions in our model.

With these analogues for the observed types of network behavior in our mind, the bifurcation diagram in Figure 7 displays several strong similarities with the detailed network model in Figure 9. For low excitation, both models exhibit regular bursting/trivial steady-state solutions. Furthermore, we see in both cases a triangular region at the bottom in which both models exhibit saturated/non-trivial steady-state solutions. Finally, we observe that the above-mentioned regions are separated by a regime of oscillatory solutions. We also note that not all types of behavior in the detailed model have a counterpart in the simplified model, but we will elaborate on this in the discussion.

## 4 Discussion

In this paper, we have studied a continuous time two-node Hopfield network with two discrete time delays. The model has been derived in [5], and it describes the activity of two excitatory neural populations located in different layers of the mammalian neocortex. Inhibitory connections are assumed to exist only between neurons within the same population, whereas excitatory connections are exclusively made between both populations. Furthermore, a bifurcation study in the same article has shown that the model is able to produce different types of behavior that correspond to a realistic 656-neuron model of neocortex as proposed in [34]. This detailed model is able to reproduce phenomena observed in *in vitro* experiments in mouse [4]. By studying the population model more thoroughly, we hope to gain a better understanding of the complex dynamics seen in the realistic 656-neuron model. In this way, new experiments for both *in silico* and *in vitro* environments can be proposed.

Even though Hopfield networks of this and similar forms have been studied thoroughly in other works, these works mainly consider changes of the dynamics under variation of the time delays. As the time lags in our model are fixed because of the physiological background, we are mainly interested in the dynamics’ dependency on connectivity parameters. As a new contribution to this field, we have focused our study of the model on varying connection strengths of excitatory and inhibitory connections.

All the bifurcations that we have identified in the model, both analytically and numerically, satisfied the non-degeneracy condition. Combined with the fact that the model depends smoothly on all parameters, all these bifurcations are structural. Hence, local variations of parameters will result in local variations of the bifurcations and the stability region. Some of the delicate bifurcation structures that we identified will be more sensitive to parameter variations, but only because of their limited separation in parameter space.

For the steady states in the model, we have analytically determined conditions in terms of the coupling parameters for which these states become unstable due to bifurcations. We have found that both the trivial and the non-trivial equilibria undergo fold as well as Hopf bifurcations. The non-trivial equilibria, however, are the solution of a transcendental equation, and therefore, we have studied these bifurcations numerically. In this manner, we have identified a region in parameter space of bi-stability in which both the trivial and a non-trivial fixed point are stable.

By studying the first Lyapunov coefficient at the Hopf bifurcations in the system, we have found both supercritical and subcritical bifurcations. Furthermore, we have analytically determined the type of bifurcating periodic solution, either symmetric (in-phase) or asymmetric (anti-phase) oscillations. The evolution of the periodic solutions arising at the Hopf bifurcations is studied numerically with continuation software. A large region in parameter space is determined in which both types of periodic solutions co-exist. Furthermore, we have identified numerous codim-2 bifurcations: cusp, generalized Hopf, zero-Hopf, Hopf-Hopf, fold-flip and both 1:1 and 1:2 resonance bifurcations. In the area where bistability exists between these different solutions, simulations have shown that the solutions often tend to the asymmetric solutions.

Combining the stability regions of the steady states and the periodic solutions, we have found a region in parameter space in which four types of stable solutions co-exist: the trivial fixed point, a non-trivial fixed point and both symmetric and asymmetric periodic solutions. Although it has been shown in [35] that small Hopfield networks can exhibit chaotic behavior, we have not found such behavior in this study.

The biological relevance of these results is, in our opinion, significant as well. We have shown that the complex bifurcation structure of the model matches with the dynamical changes seen in a biologically relevant model for variations of both excitatory and inhibitory strengths [4]. This relation is most clear for the regular bursting, oscillatory and saturated states of the detailed model because these have a clear equivalent attractor in the population model studied in this article. The other states of the detailed network, however, might be produced by the population model as part of a transient behavior. Long transients, during which the model resides close to several attractors for an extended period of time, are not uncommon for multi-stable delayed systems. Since these transients are not attractors themselves, they cannot be identified with a bifurcation analysis as in this study. Therefore, it remains to be determined whether the population model exhibits long transients and, if so, whether the time-series correspond with the two missing network states, *i.e.*, desynchronized and irregular bursting as described in [4].

Although the considered model has very little resemblance with the structures of a real brain, we still believe that studying models like these provide new insights. Complex bifurcation structures and multi-stability observed in these models reveal possible transitions of network behavior that might not have been considered before. For that reason, we plan to seek and analyze such critical transitions more accurately with a detailed model of neuronal activity.

Furthermore, we plan to investigate networks of similar systems in order to study emergent patterns. It is promising that the combined analytical/numerical study of a single column already shows interesting dynamics, in particular, multi-stability. We expect to find patterns in such networks that will be relevant to understand observed patterns in slice experiments.

## Declarations

### Acknowledgements

The authors thank Odo Diekmann, Wim van Drongelen and Sebastiaan Janssens for giving helpful directions. The first author is supported by The Netherlands Organization of Scientific Research NWO, grant no. 635.100.019, ‘From Spiking Neurons to Brain Waves’.

## Authors’ Affiliations

## References

- Lytton WW:
**Computer modelling of epilepsy.***Nat Rev, Neurosci*2008,**9:**626–637. 10.1038/nrn2416View ArticleGoogle Scholar - Wendling F, Bartolomei F, Bellanger JJ, Chauvel P:
**Epileptic fast activity can be explained by a model of impaired GABAergic dendritic inhibition.***Eur J Neurosci*2002,**15:**1499–1508. 10.1046/j.1460-9568.2002.01985.xView ArticleGoogle Scholar - Lopes da Silva FH, Blanes W, Kalitzin SN, Parra J, Suffczynski P, Velis DN:
**Epilepsies as dynamical diseases of brain systems: basic models of the transition between normal and epileptic activity.***Epilepsia*2003,**44:**72–83. 10.1111/j.0013-9580.2003.12005.xView ArticleGoogle Scholar - Van Drongelen W, Lee HC, Hereld M, Chen Z, Elsen FP, Stevens RL:
**Emergent epileptiform activity in neural networks with weak excitatory synapses.***IEEE Trans Neural Syst Rehabil Eng*2005,**13:**236–241. 10.1109/TNSRE.2005.847387View ArticleGoogle Scholar - Visser S, Lee HC, Meijer HGE, van Drongelen W, van Putten MJAM, van Gils SA:
**Comparing epileptiform behavior of meso-scale detailed models and populations models of neocortex.***Clin Neurophysiol*2010,**27:**471–478. 10.1097/WNP.0b013e3181fe0735View ArticleGoogle Scholar - Baldi P, Atiya A:
**How delays affect neural dynamics and learning.***IEEE Trans Neural Netw*1994,**5**(4):612–621. 10.1109/72.298231View ArticleGoogle Scholar - Bélair J, Campbell SA:
**Stability and bifurcations of equilibria in a multiple-delayed differential equation.***SIAM J Appl Math*1994,**54:**1402–1424. 10.1137/S0036139993248853MathSciNetView ArticleGoogle Scholar - Campbell SA, Edwards R, Van den Driessche P:
**Delayed coupling between two neural network loops.***SIAM J Appl Math*2004,**65:**316–335. 10.1137/S0036139903434833MathSciNetView ArticleGoogle Scholar - Campbell SA, Yuan Y, Bungay SD:
**Equivariant Hopf bifurcation in a ring of identical cells with delayed coupling.***Nonlinearity*2005,**18:**2827–2846. 10.1088/0951-7715/18/6/022MathSciNetView ArticleGoogle Scholar - Campbell SA, Ncube I, Wu J:
**Multistability and stable asynchronous periodic oscillations in a multiple-delayed neural system.***Physica D, Nonlinear Phenom*2006,**214**(2):101–119. 10.1016/j.physd.2005.12.008MathSciNetView ArticleGoogle Scholar - Chen Y, Wu J:
**Minimal instability and unstable set of a phase-locked periodic orbit in a delayed neural network.***Physica D, Nonlinear Phenom*1999,**134**(2):185–199. 10.1016/S0167-2789(99)00111-6View ArticleGoogle Scholar - Faria T:
**On a planar system modelling a neuron network with memory.***J Differ Equ*2000,**168:**129–149. 10.1006/jdeq.2000.3881MathSciNetView ArticleGoogle Scholar - Guo S, Huang L:
**Hopf bifurcating periodic orbits in a ring of neurons with delays.***Physica D, Nonlinear Phenom*2003,**183**(1–2):19–44. 10.1016/S0167-2789(03)00159-3MathSciNetView ArticleGoogle Scholar - Guo S, Chen Y, Wu J:
**Two-parameter bifurcations in a network of two neurons with multiple delays.***J Differ Equ*2008,**244**(2):444–486. 10.1016/j.jde.2007.09.008MathSciNetView ArticleGoogle Scholar - Huang L, Wu J:
**Nonlinear waves in networks of neurons with delayed feedback: pattern formation and continuation.***SIAM J Math Anal*2003,**34**(4):836–860. 10.1137/S0036141001386519MathSciNetView ArticleGoogle Scholar - Olien L, Bélair J:
**Bifurcations, stability, and monotonicity properties of a delayed neural network model.***Physica D, Nonlinear Phenom*1997,**102**(3–4):349–363. 10.1016/S0167-2789(96)00215-1View ArticleGoogle Scholar - Shayer LP, Campbell SA:
**Stability, bifurcation, and multistability in a system of two coupled neurons with multiple time delays.***SIAM J Appl Math*2000,**61:**673–700. 10.1137/S0036139998344015MathSciNetView ArticleGoogle Scholar - Wei J, Ruan S:
**Stability and bifurcation in a neural network model with two delays.***Physica D, Nonlinear Phenom*1999,**130**(3–4):255–272. 10.1016/S0167-2789(99)00009-3MathSciNetView ArticleGoogle Scholar - Wei JJ, Velarde MG:
**Bifurcation analysis and existence of periodic solutions in a simple neural network with delays.***Chaos*2004,**14**(3):940–953. 10.1063/1.1768111MathSciNetView ArticleGoogle Scholar - Wu J, Faria T, Huang YS:
**Synchronization and stable phase-locking in a network of neurons with memory.***Math Comput Model*1999,**30**(1–2):117–138. 10.1016/S0895-7177(99)00120-XMathSciNetView ArticleGoogle Scholar - Yuan Y, Campbell SA:
**Stability and synchronization of a ring of identical cells with delayed coupling.***J Dyn Differ Equ*2004,**16:**709–744. 10.1007/s10884-004-6114-yMathSciNetView ArticleGoogle Scholar - Yuan Y:
**Dynamics in a delayed-neural network.***Chaos Solitons Fractals*2007,**33**(2):443–454. 10.1016/j.chaos.2006.01.018MathSciNetView ArticleGoogle Scholar - Wen UP, Lan KM, Shih HS:
**A review of Hopfield neural networks for solving mathematical programming problems.***Eur J Oper Res*2009,**198**(3):675–687. 10.1016/j.ejor.2008.11.002MathSciNetView ArticleGoogle Scholar - Wilson HR, Cowan JD:
**Excitatory and inhibitory interactions in localized populations of model neurons.***Biophys J*1972,**12:**1–24.View ArticleGoogle Scholar - Coombes S, Laing C:
**Delays in activity-based neural networks.***Philos Trans R Soc Lond Ser A, Math Phys Sci*2009,**367:**1117–1129. 10.1098/rsta.2008.0256MathSciNetView ArticleGoogle Scholar - Song Y, Makarov VA, Velarde MG:
**Stability switches, oscillatory multistability, and spatio-temporal patterns of nonlinear oscillations in recurrently delay coupled neural networks.***Biol Cybern*2009,**101:**147–167. 10.1007/s00422-009-0326-5MathSciNetView ArticleGoogle Scholar - Bellman R, Cooke KL:
*Differential-Difference Equations*. Academic Press, San Diego; 1963.Google Scholar - Hale JK:
*Functional Differential Equations*. Springer, Berlin; 1971.View ArticleGoogle Scholar - Mahaffy JM, Zak PJ, Joiner KM:
**A geometric analysis of stability regions for a linear differential equation with two delays.***Int J Bifurc Chaos Appl Sci Eng*1995,**5**(3):779. 10.1142/S0218127495000570MathSciNetView ArticleGoogle Scholar - Diekmann O, van Gils SA, Verduyn-Lunel SM, Walther HO:
*Delay Equations: Functional-, Complex-, and Nonlinear Analysis*. Springer, New York; 1995.View ArticleGoogle Scholar - Engelborghs K, Luzyanina T, Roose D:
**Numerical bifurcation analysis of delay differential equations using DDE-BIFTOOL.***ACM Trans Math Softw*2002,**28:**1–21. 10.1145/513001.513002MathSciNetView ArticleGoogle Scholar - Roose D, Szalai R:
**Continuation and bifurcation analysis of delay differential equations.**In*Numerical Continuation Methods for Dynamical Systems: Path Following and Boundary Value Problems*. Springer, Berlin; 2007:359–399.View ArticleGoogle Scholar - Kuznetsov YA:
*Elements of Applied Bifurcation Theory*. Springer, New York; 2004.View ArticleGoogle Scholar - Van Drongelen W, Koch H, Elsen FP, Lee HC, Mrejeru A, Doren E, Marcuccilli CJ, Hereld M, Stevens RL, Ramirez JM:
**Role of persistent sodium current in bursting activity of mouse neocortical networks in vitro.***J Neurophysiol*2006,**96:**2564–2577. 10.1152/jn.00446.2006View ArticleGoogle Scholar - Lu H:
**Chaotic attractors in delayed neural networks.***Phys Lett A*2002,**298**(2–3):109–116. 10.1016/S0375-9601(02)00538-8View ArticleGoogle Scholar

## Copyright

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.